HPLC 3

Non-microscopic Characterization
Category General
Difficulty Level Normal
Audience Level General
Description Optical microscopy, Scanning probe microscopy and Electron microscopy are known as microscopic techniques. Other methods such as Spectrophotometry, Chromatography, BET and etc. are classified as non-microscopic characterization. Non-microscopic characterization is incorporated in this course.
Overview
The term “non-microscopic” is used when Optical microscopy, Scanning probe microscopy and Electron microscopy are not used to determine materials’ quantity and quality. Pieces of information such as what element and how much of it are in a component, specific surface area and etc. are determined using non-microscopic methods.


Booklet
Description
 This is the third lesson of the four lessons about HPLC, it continues the talk and goes through some definitions for example the detectors, mobile phase in the liquid chromatography, different kinds of chromatography based on relative polarities of the mobile and the still phase, partition chromatography and its types.
Mental Challenge
 Which kind of Chromatography has the highest application between HPLCs? Why?
Quizzes and Activities
 Activity: Find or prepare a catalog of different types of detectors, including their main properties. (image you want to compare and buy one of them).
Further Reading Sources

1.https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/high-performance-liquid-chromatography

2.https://www.shodex.com/en/kouza/f.html#!